MTT|Thiazolyl Blue Tetrazolium Bromide 噻唑蓝

规格: 98%
CAS: 298-93-1
产品编号: H11402
MDL: MFCD00011964
品牌: INFI
Chemical NameThiazolyl Blue Tetrazolium Bromide
MDL NumberMFCD00011964
CAS Number298-93-1
Reaxys-RN3825277
PubChem Substance ID87567423
Synonym Thiazolyl Blue {LY} Thiazolyl Blue {} {LY} Thiazolyl Blue {} {} {LY} Thiazolyl Blue {} {} {} {LY} Thiazolyl Blue {} {} {} {} {LY} Thiazolyl Blue {} {} {} {} {} {LY} Thiazolyl Blue {} {} {} {} {} {} {LY} Thiazolyl Blue {} {} {} {} {} {} {} {LY} Thiazolyl Blue {} {} {} {} {} {} {} {} {LY} Thiazolyl Blue {} {} {} {} {} {} {} {} {} {LY} Thiazolyl Blue {} {} {} {} {} {} {} {} {} {} {LY} Thiazolyl Blue {} {} {} {} {} {} {} {} {} {} {} {LY} Thiazolyl Blue {} {} {} {} {} {} {} {} {} {} {} {} {LY} Thiazolyl Blue {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Thiazolyl Blue {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Thiazolyl Blue {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Thiazolyl Blue {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} Thiazolyl Blue {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {
EC Number206-069-5
Beilstein Registry Number4081397
Chemical Name Translation噻唑蓝溴化四唑
Wiswesser Line NotationT5NNKNJ BR& ER& C- BT5N CSJ D1 E1 &E
LabNetwork Molecule IDLN00197171
Canonical SMILESCC1=C(C)N=C(N2N=C(C3=CC=CC=C3)N=[N+]2C4=CC=CC=C4)S1.[Br-]
GHS Symbol
TYPE OF TEST            : Mutation in microorganisms
TEST SYSTEM             : Bacteria - Salmonella typhimurium
DOSE/DURATION           : 5 ug/plate
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 68,107,1979

TYPE OF TEST            : Mutation in microorganisms
TEST SYSTEM             : Bacteria - Escherichia coli
DOSE/DURATION           : 5 ug/plate
REFERENCE :
   MUREAV Mutation Research.  (Elsevier Science Pub. B.V., POB 211, 1000 AE
   Amsterdam, Netherlands) V.1-    1964-  Volume(issue)/page/year: 68,107,1979

RTECSXF8060000
Safety Statements
  • S22 Do not breathe dust 不要吸入粉尘;
  • S24/25 Avoid contact with skin and eyes 避免皮肤和眼睛接触;
  • S26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice 眼睛接触后,立即用大量水冲洗并征求医生意见;
  • S36/37 Wear suitable protective clothing and gloves 穿戴适当的防护服和手套;
  • S36/37/39 Wear suitable protective clothing, gloves and eye/face protection 穿戴适当的防护服、手套和眼睛/面保护;
  • S37 Wear suitable gloves 戴适当手套;
  • S60 This material and its container must be disposed of as hazardous waste 该物质及其容器必须作为危险废物处置;
    Risk Statements
    • R36/37/38 Irritating to eyes, respiratory system and skin 对眼睛、呼吸系统和皮肤有刺激性
    • R68 Possible risk of irreversible effects 可能有不可挽回的作用的危险
      Storage condition 2-8°C, sealed storage, away from moisture and light {LY} 2-8°C, sealed storage, away from moisture and light 2-8℃避光保存。 Light Sensitive Light, Air & Moisture Sensitive {} {LY} 2-8°C, sealed storage, away from moisture and light {} {} {LY} 2-8°C, sealed storage, away from moisture and light {} {} {} {LY} 2-8°C, sealed storage, away from moisture and light {} {} {} {} {LY} 2-8°C, sealed storage, away from moisture and light {} {} {} {} {} {LY} 2-8°C, sealed storage, away from moisture and light {} {} {} {} {} {} {LY} 2-8°C, sealed storage, away from moisture and light {} {} {} {} {} {} {} {LY} 2-8°C, sealed storage, away from moisture and light {} {} {} {} {} {} {} {} {LY} 2-8°C, sealed storage, away from moisture and light {} {} {} {} {} {} {} {} {} {LY} 2-8°C, sealed storage, away from moisture and light {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {LY} 2-8°C, sealed storage, away from moisture and light {} {} {} {} {} {} {} {} {}
      WGK Germany3
      Hazard statements
      • H315 Causes skin irritation 会刺激皮肤
      • H341 Suspected of causing genetic defects 怀疑导致遗传性缺陷
      • H319 Causes serious eye irritation 严重刺激眼睛
      • H335 May cause respiratory irritation 可能导致呼吸道刺激
        Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
        Precautionary statements
        • P261 Avoid breathing dust/fume/gas/mist/vapours/spray. 避免吸入粉尘/烟/气体/烟雾/蒸汽/喷雾。
        • P280 Wear protective gloves/protective clothing/eye protection/face protection. 戴防护手套/防护服/眼睛的保护物/面部保护物。
        • P281 Use personal protective equipment as required. 使用所需的个人防护装备。
        • P305+P351+P338
          Signal word Warning
          Hazard Codes Xi Xn
        • 常用于基于MTT检测方法的细胞活力、细胞增殖或细胞毒性检测。因为MTT具有正电荷,它可以穿过细胞膜并被线粒体还原形成紫色甲臜染料。溶解MTT甲臜染料需要使用有机溶剂。脱氢酶的检测,染色法。
        • {SA}
        • 1. Mosmann, T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65 , 55-63, (1983)
        • {SA}
        • 2. Rastinejad., F., et al., Tumor suppression by RNA from the 3' untranslated region of α-tropomyosin. Cell 75 , 1107-17, (1993) 摘要
        • {SA}
        • {SA} 3. Denizot, F., and Lang, R., Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Methods 89 , 271-7, (1986) 摘要
        • {SA}
        • {SA} 4. Carmichael, J., et al., Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47 , 936-42, (1987)
        • {SA}
        • 5. Boyle, V.J., et al., Rapid, modified Kirby-Bauer susceptibility test with single, high-concentration antimicrobial disks. Antimicrob. Agents Chemother. 3 , 418-24, (1973) 摘要
        • {SA}
        • {SA} 6. Pearse, A.G.E., and Hess, R., Subtantivity and other factors responsible for formazan patterns in dehydrogenase histochemistry. Experientia 17 , 136, (1961) 摘要
        • {SA}
        • {SA} 7. Bernofsky, C., and Swan, M., An improved cycling assay for nicotinamide adenine dinucleotide. Anal. Biochem. 53 , 452-8, (1973) 摘要
        • {SA}
        • {SA} 8. Hess, R., et al., Cytochemical localization of pyridine nucleotide-linked dehydrogenases. Nature 181 , 1531-2, (1958) 摘要
        • {SA}
        • {SA} 9. Pearse, A.G., Intracellular localisation of dehydrogenase systems using monotetrazolium salts and metal chelation of their formazans. J. Histochem. Cytochem. 5 , 515-27, (1957) 摘要
        • {SA}
        • {SA} Perri M, Pingitore A, Cione E, et al., Proliferative and anti-proliferative effects of retinoic acid at doses similar to endogenous levels in Leydig MLTC-1/R2C/TM-3 cells. Biochim. Biophys. Acta 1800 , 993-1001, (2010) 摘要
        • {SA}
        • {SA} Beil. 27 ,IV,6045
        • {SA}
        • 298-93-1 H11402 MTT|Thiazolyl Blue Tetrazolium Bromide
噻唑蓝

          化学属性

          Mol. FormulaC18H16BrN5S
          Mol. Weight414.32
          Melting Point195°C
          TSCAYes
          Density1.422
          Stability对光和湿度敏感
          SolubilitySoluble in water (20 mg/ml), ethanol (20 mg/ml), 2-methoxyethanol (20 mg/ml), buffered salt solutions (5 mg/ml), culture media (5 mg/ml), and PBS.
          Appearance powder

          *以上化合物性质及应用等信息仅供参考